rongsheng biotech pricelist

Rattanpal, H.S., Kaur, G., and Gupta, M. (2011). In vitro plant regeneration in rough lemon (Citrus jambhiri Lush) by direct organogenesis. African Journal of Biotechnology 10,13724-13728.

Reyes CA, De Francesco A, Pena EJ, Costa N, Plata MI, Sendin L, Castagnaro AP, Garcia ML. Resistance to Citrus psorosis virus in transgenic sweet orange plants is triggered by coat protein-RNA silencing. Journal of Biotechnology 2011;151(1):151-158.

Wang J, Liu JH, Kurosawa T, Nada K, Ban Y, Moriguchi T. Cloning, biochemical identification, and expression analysis of a gene encoding S-adenosylmethionine decarboxylase in navel orange (Citrus sinensis Osbeck). Journal of Horticultural Science & Biotechnology 2010;85(3):219-226.

Cervera M, Navarro L, Pena L. Gene stacking in 1-year-cycling APETALA1 citrus plants for a rapid evaluation of transgenic traits in reproductive tissues. Journal of Biotechnology 2009;140(3-4):278-282.

Omar, A. A., Dekkers, M.G.H., Graham, J.H., & Grosser, J.W. (2008). Estimation of transgene copy number in transformed citrus plants by auantitative multiplex real-time PCR. Biotechnology Progress, 24 (6), 1241-1248.

Omar, A. A., Song, W. Y., & Grosser, J. W. (2007). Introduction of Xa21, a Xanthomonas-resistance gene from rice, into "Hamlin" sweet orange [Citrus sinensis (L.) Osbeck] using protoplast-GFP co-transformation or single plasmid transformation. Journal of Horticultural Science and Biotechnology, 82 (6), 914-923.

Genomic DNA was extracted from 2 mL suspension when the isolates were cultured to the logarithmic stage (after about 12 h of culturing), using the TIANamp Bacterial DNA Kit (TianGen Biotech, China). The quality and quantity characterization of the genomic DNA of representative isolates were presented in Supplementary Table S1.1 To acquire the taxonomic identities of sequences, taxonomy was assigned using BLAST in NCBI (https://blast.ncbi.nlm.nih.gov/), and the sequences of 16S rRNA were submitted to NCBI. After 16S rRNA sequencing, the strains with same 16S rRNA sequences (identity 100%) were defined as one group, and 3–5 strains were randomly selected from each group for functional gene polymerase chain reaction (PCR) amplification. The nitrate reductase genes (narG and napA) and nitrite reductase genes (nirS, nirK, and nrfA) were performed to confirm whether the isolates have nitrate-reducing genes, and whether they have pathways to further reduce the nitrite produced via nitrate reduction. The primers and amplified conditions for 16S rRNA, narG, napA, nirS, nirK, and nrfA were presented in Supplementary Table S2.1 Each functional gene was amplified with two or three pairs of primers to determine its presence. As long as the product amplified by any pair of primers of one gene had a unique band in the right place, it was assumed to contain the gene. The PCR amplification was performed in a total volume of 25 μL, containing DNA 50 ng, 2× Taq mix (TransGen Biotech, China) 12.5 μL, forward primer (10 μmol·L−1) 1 μL, and reverse primer (10 μmol·L−1) 1 μL; finally, nuclease-free water was added to 25 μL. The PCR products were detected using electrophoresis on 1.0% agarose gels.Determination of growth rate and nitrite accumulation