rongsheng cai md in stock
There is a star rating of 4/5 for Dr. Rongsheng Cai, MD. Patients say that they trusted the provider"s decisions and the provider explained conditions well. See all patient feedback on Sharecare.
Dr. Rongsheng Cai is a Neurosurgery Specialist in Tulsa, Oklahoma. He graduated with honors in 1985. Having more than 38 years of diverse experiences, especially in NEUROSURGERY, Dr. Rongsheng Cai affiliates with no hospital, cooperates with many other doctors and specialists in medical group University Of Arkansas. Call Dr. Rongsheng Cai on phone number (918) 494-1710 for more information and advice or to book an appointment.
This doctor profile was extracted from the dataset publicized on Feb 1st, 2018 by the Centers for Medicare and Medicaid Services (CMS) and from the corresponded NPI record updated on Jun 2nd, 2020 on NPPES website. If you found out anything that is incorrect and want to change it, please follow this Update Dataguide.
Dr. Rongsheng Cai M.D. is a male health care provider in Tulsa with Neurological Surgeon listed as his primary medical specialization. His credentials are: M.D. (Doctor of Medicine). Dr. Rongsheng Cai M.D."s practice location is: 6151 S Yale Ave Ste 2403 Tulsa, OK 74136-1907.
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Correspondence to: Dr Rongsheng Zeng or Dr Lusai Xiang, Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, 56 Lingyuanxi Road, Guangzhou, Guangdong 510055, P.R. China, E-mail: moc.361@4270xoag, E-mail: nc.ude.usys.liam@slgnaix
Expression of osteoprotegerin, the osteogenic marker osteocalcin, and the odontogenic marker dentin sialoprotein (DSP) was assessed by immunohistochemical (IHC) staining. Anti-osteoprotegerin polyclonal antibody (5 µg/ml; cat. no. AF459; R&D Systems), anti-osteocalcin polyclonal antibody (50 µl/ml; cat. no. ab93876; Abcam) and anti-DSP monoclonal antibody (2 µg/ml; cat. no. MABT37; EMD Millipore) were used as primary antibodies. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL; cat. no. ab206386; Abcam) staining was carried out according to manufacturer"s instructions to evaluate apoptosis activity in tooth germs with/without osteoprotegerin treatment.
Expression pattern of OPG. (A and B) Volcano plot and heatmap of differential protein expression comparing extracellular protein levels between tooth germs and samples of isolated dental mesenchyme. OPG is indicated in the figures. In the volcano plot, the log2 fold change was calculated by comparing tooth germs vs. the dental mesenchyme. Microarray analysis for tooth germs and the dental mesenchyme was performed in triplicate. (C) Spatial-temporal pattern of OPG expression during tooth development. At first, OPG expression was confined to the dental epithelium (a1 and a2). Then, OPG expression in the dental mesenchyme gradually increased as development proceeded (b-f). (C) The red arrows indicate parts of the tissue that were positive for immunohistochemical staining. Scale bar, 50 µm. OPG, osteoprotegerin; TG, tooth germ; ME, dental mesenchyme; am, ameloblasts; d, dentin; de, dental epithelium; dm, dental mesenchyme; md, mandible; od, odontoblast.
Injection grade hydroxypropyl-β-cyclodextrin (HP-βCD, Fw = 1541.54, purity ≥ 98%) was purchased from Zhiyuan Biotechnology Co., Ltd. (China). Anhydrous N,N-dimethylformamide (DMF), anhydrous dichloromethane (DCM) and N,N"-carbonyldiimidazole (CDI) were obtained from J&K (China). 4-(Hydroxymethyl)phenylboronic acid (PBA) and 4-acetamidophenol (APAP) were supplied by Sigma-Aldrich (St. Louis, MO, USA). Rifampicin (RFP) was purchased from Tokyo Chemical Industry Co., Ltd. (Japan). Baicalein (BE) was purchased from MedChemExpress (purity > 98%). Cyanine5 NHS ester (Cy5) and Cyanine7.5 NHS ester (Cy7.5) were obtained from Lumiprobe (USA). William"s E Medium, Dulbecco"s Modified Eagle"s Medium (DMEM), fetal bovine serum (FBS), penicillin, streptomycin and trypsin for cell culture were purchased from Gibco (USA). FITC Annexin V Apoptosis Detection Kits with 7-aminoactinomycin D (#640922) was purchased from Biolegend (USA), while MTT Cell Proliferation and Cytotoxicity Assay Kit (#C0009), Enhanced BCA Protein Assay Kit (#P0010S) and Reactive Oxygen Species Assay Kit based on DCFH-DA (#S0033) were supplied by Beyotime Biotechnology Co. Ltd. (China). N-(4-Diphenylphosphinophenyl)-N"-(3, 6, 9, 12-tetraoxatridecyl)perylene-3, 4, 9, 10-tetracarboxydiimide (LiperFluo™, #L248) was obtained by Dojindo Molecular Technologies (Japan). TMRE-Mitochondrial Membrane Potential Assay Kit (#ab113852) was obtained from Abcam (USA). Collagenase Ⅱ, DNAse I, Lymphoprep and D-Hank"s were purchased from Solarbio (China). APC-labeled anti-mouse Ly6G antibody (#127613) and FITC-labeled anti-mouse CD11b antibody (#101205) were purchased from Biolegend (USA). With the exception of myeloperoxidase (MPO) ELISA kits (#E4580) was obtained from BioVision (USA), glutathione (GSH), glutathion reductases (GR), malondialdehyde (MDA), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) assay kit were purchased from Nanjing Jiancheng Bioengineering Institute (China). QuantiCyto® Mouse ELISA kits for TNF-α and IL-1β were purchased from Neobioscience (China). Kits for H2O2 (#DIOX-250) were purchased from BioAssay Systems (USA). All the other reagents are commercially available and used as received.
After administrated with different samples for 18 h, mice were euthanized and livers were isolated and weighed. The ratio of liver tissue to mouse body weight was calculated as the liver index. Additionally, liver tissues were homogenized in cold PBS with a tissue-tearor homogenizer and then centrifuged at 16,000 g for 10 min at 4 °C. The resulting supernatants were collected for quantification of the levels of oxidative mediators (H2O2, GSH, MDA, MPO) by commercial kits and inflammatory mediators (TNF-α and IL-1β) by ELISA. The total protein was measured by BCA kit.