rongsheng wang temple manufacturer

Zhang Y, Fang C,Wang RE, Wang Y, Guo H, Guo C, Zhao L, Li S, Li X, Schultz PG, Cao YJ, Wang F. Proc Natl Acad Sci U S A. 2019 Aug 6;116(32):15889-15894.

Wissler HL, Ehlerding EB, Lyu Z, Zhao Y, Zhang S, Eshraghi A, Buuh ZY, McGuth JC, Guan Y, Engle JW, Bartlett SJ, Voelz VA, Cai W,Wang RE. Mol Pharm. 2019 May 6;16(5):2028-2036.

For evaluation of Axin peptide analogues, DLD-1 cells were cultured in 96-well flat bottom plates up to 70–80% confluency and were transfected with 200 ng M50 TOPFlash plasmid (a gift from Professor Raymond Habas, Temple Biology) and 50 ng pRL-TK plasmid (Promega). 12 h after transfection, cells were treated with DMSO vehicle or 15 μM of Axin peptide analogues for another 24 h. Luciferase activity was measured using the Dual-Glo luciferase assay kit (Promega) on a Biotek Synergy H1 plate reader using the Gen5 software. Data were processed as firefly: Renilla luciferase ratio for each control or treatment group, and were further normalized against the vehicle control group (100%) as the Relative Activities.

HONG KONG, Nov 26 (Reuters) - China Rongsheng Heavy Industries Group, the country’s largest private shipbuilder, said its chairman had stepped down just three months after the company posted its sharpest fall in half-year net profit.

Listed in November 2010, Rongsheng was hit by an insider dealing scandal involving a firm owned by Zhang ahead of the $15.1 billion bid for Canadian oil firm Nexen Inc by China offshore oil and gas producer CNOOC.

Rongsheng said earlier this month that investment firm Well Advantage, controlled by Zhang, had agreed to pay $14 million as part of a settlement deal with the U.S. Securities and Exchange Commission (SEC).

In August, Rongsheng posted an 82 percent drop in half-year profit on a dearth of new orders and warned economic uncertainties would continue to weigh on the global shipping market.

As part of the changes at China Rongsheng, the company said that Zhang De Huang was retiring and had resigned as an executive director and as vice chairman of the board.

HONG KONG, July 5 (Reuters) - China Rongsheng Heavy Industries Group, China’s largest private shipbuilder, appealed for financial help from the Chinese government and big shareholders on Friday after cutting its workforce and delaying payments to suppliers.

Hours after China Rongsheng made its appeal in a filing to the Hong Kong stock exchange, where the company is listed, Beijing vowed to bring about the orderly closure of some factories in industries plagued by overcapacity.

The statement by the State Council, or cabinet, laid out broad plans to ensure banks support the kind of economic rebalancing Beijing wants as it looks to focus more on high-end manufacturing. It did not mention any specific industries or companies and there was no suggestion it was referring to Rongsheng.

China Rongsheng said it was expecting a net loss for the six months that ended June 30 from a year earlier, according to the filing. It gave no figures.

Rongsheng shares plunged 16 percent to a record low in heavy turnover on Friday, leaving its market capitalisation at just under $1 billion. The Hang Seng Index climbed 1.9 percent. China Rongsheng is down 28.2 percent on the year.

In its filing, China Rongsheng said some workers had been made redundant, although it gave no numbers or timeframe for the losses. The company did not immediately respond to requests for more information.

China Rongsheng has said it won only two shipbuilding orders worth $55.6 million last year when its target was $1.8 billion worth of contracts. This year, it received orders to build two drilling rigs used in oil exploration, worth $360 million.

While the Chinese shipbuilding industry faced “unprecedented challenges”, China Rongsheng’s board was confident management could ease pressure on working capital in the near future and maintain normal operations, the company said in the filing.

According to its December 2012 annual report, issued on March 26, China Rongsheng’s cash and cash equivalents fell to 2.1 billion yuan from 6.3 billion yuan a year ago.

“The group is ... actively seeking financial support from the government and the substantial shareholders of the company, and increasing its efforts in negotiations with its customers to maximise the collection of receivables,” China Rongsheng said in the filing.

A note from Macquarie Equities research said the statement highlighted the “severity” of China Rongsheng’s liquidity problems, adding this was not necessarily representative of the wider sector.

It said other listed Chinese shipyards were not as leveraged as China Rongsheng. The loan from Zhang was a surprise, it said, showing how badly the company needed cash.

“Rongsheng will need to address the problems immediately to reassure the market,” said Martin Rowe, managing director of Clarkson Asia Limited, a global shipping services provider.

I am retaking Orgo1 for this semester. Compared to the previous lecturer, Dr Wang makes 2201 bearable and I enjoyed most of his lectures. Orgo is always tough and overwhelming (the nature of this subject). But Dr. Wang is very organized and strictly follows the book. His test questions came directly from the lecture notes and book problems.

Using olefin-containing unnatural amino acids and RCM mediated chemical crosslinking, Grubbs, Verdine, Walensky, and others have done seminal work to develop RCM-stapled peptides as a promising class of therapeutics (Blackwell HE et al., 1998, Angew Chem Int Ed Engl, 37:3281-3284; Walensky LD et al., 2004, 2004, Science, 305:1466-1470; Grossmann TN et al., 2012, Proc Natl Acad Sci USA, 109:17942-17947; Bird GH et al., 2014, ACS Chem Biol, 9:831-837; Chapuis H et al., 2012, Amino Acids, 43:2047-2058; Cromm PM et al., 2015, ACS Chem Biol, 10:1362- 1375; Walensky LD et al., 2014, J Med Chem, 57:6275-6288). The hydrocarbon stapled peptides not only maintained the desired tertiary structures and the associated targeting specificities, but also possessed increased binding affinity and protease resistance (Wan J et al., 2016, Lead Generation, 259-306; Bird GH et al., 2014, ACS Chem Biol, 9:831- 837; Walensky LD et al., 2014, J Med Chem, 57:6275-6288). One hydrocarbon stapled peptide, a lead p53 mimic (ALRN-6924), has been in clinical testing to treat a diverse set of tumors including acute myeloid leukemia (AML) (Scott DE et al., 2016, Nat. Rev. Drug Discov., 15:533-550). Yet, RCM-based stapling necessitates the use of metal-based catalysts that were sometimes incompatible with other functional groups present in peptides such as a thiourea moiety (Verdine GL et al., 2012, Methods Enzymok, 503:3- 33). The increased hydrophobicity brought by hydrocarbon linkers may also incur issues in targeting specificity and aqueous solubility(Verdine GL et al., 2012, Methods Enzymok, 503:3-33). To date, a number of other stapling strategies based on different crosslinking chemistry have been established (Tian Y et al., 2016, Chem. Sci., 7:3325- 3330; Lau YH et al., 2015, Chem. Soc. Rev., 44:91-102; Muppidi A et al., 2012, J Am Chem Soc, 134:14734-14737; Kumita JR et al., 2000, Proc Natl Acad Sci USA, 97:3803- 3808; Jo H et al., 2012, J Am Chem Soc, 134:17704-17713; Wang Y et al., 2015, Angew Chem Int Ed Engl, 54:10931-10934; Mendive-Tapia L et al., 2015, Nat Commun,

Subsequent studies then focused on the evaluation of the FTDR-based cyclization on varied peptides of interesting chemical and biological properties. The Axin mimetic analogue first caught attention as it was a classic peptide used for stapling (Grossmann TN et al., Proc Natl Acad Sci USA, 109:17942-17947; Wang Y et al., 2015, Angew Chem Int Ed Engl, 54: 10931-10934), and its blocking of the Axin- b-catenin PPI has been shown to inhibit the Wnt signaling pathway that is crucial for the development of colorectal cancers and acute myeloid leukemia (Grossmann TN et al., Proc Natl Acad Sci USA, 109:17942-17947; Ysebaert L et al., 2006, Leukemia, 20:1211-1216; Wang Y et al., 2010, Science, 327:1650-1653; Chung EJ et al., 2002, Blood, 100:982-990). A pair of fluoroacetamide-containing amino acids with a combination of possible chirality (XL or XD) were incorporated into the i and i+4 positions to render the fifteen amino acid long fluoroacetamide containing Axin analogues (SEQ ID NO: 5) (XL,XL for peptide (20), XD,XD for (27), XL,XD for (34), and XD,XL for (38)) (Figure 50A and Figure 50B). With the optimized reaction conditions, FTDR-based macrocyclization were investigated on

Despite intensive studies, the uptake mechanisms for cell-penetrating peptides remained ambiguous and largely varied due to their complicated nature (Silhol M et al., 2002, Eur J Biochem, 269:494-501; Deshayes S et al., 2005, Cell Mol Life Sci, 62:1839-1849; Kaplan IM et al., 2005, J Control Release, 102:247-253). Small molecule inhibitors specific for each endocytic pathway have been routinely utilized to interrogate the cellular uptake mechanisms of many transporters (Halvorsen B et al., 1998, Biochem J, 331:743-752; Wang LH et ak, 1993, J Cell Biol, 123:1107-1117; Gottlieb TA et al., 1993, J Cell Biol, 120:695-710; Zhu XD et al., 2011, J Biol Chem, 286:8231-8239). For example, nystatin as a sterol-binding agent was used to selectively block caveolin- dependent endocytosis (Zhu XD et al., 2011, J Biol Chem, 286:8231-8239, while chlorpromazine selectively inhibited clathrin-mediated endocytosis (Wang LH et al., 1993, J Cell Biol, 123 : 1107-1117; Zhu XD et al., 2011, J Biol Chem, 286:8231-8239). Cytochalasin D specifically induced depolymerization of actin and ceased the subsequent

Molecular Dynamics Simulation: Molecular dynamics (MD) simulations of stapled/unstapled Axin peptides and HIV peptides were performed using the OpenMM 7.0 simulation package (Eastman P et al., 2017, PLOS Computational Biology, 13:el005659) on the Folding@home distributed computing platform (Shirts M et al., 2000, Science, 290:1903-1904). The AMBER ffl4SB force field (Maier JA et al, 2015, Journal of Chemical Theory and Computation, 11 :3696-3713) was used for the peptide residues, while non-natural residues and linkers used GAFF (Wang J et al., 2004, Journal of Computational Chemistry, 25: 1157-1174) with partial charges from AMl-BCC (Jakalian A et al., 2002, Journal of Computational Chemistry, 23:1623-1641), parameterized using the antechamber package of AmberToolsl7 (Case DA et al., 2017 AMBER, University of California, San Francisco). Axin peptide simulations were initiated from helical conformations taken from crystal structure PDB:1QZ7. These structures were solvated in a ~ (55 A)3 cubic periodic box with TIP3P water molecules and Na+ and CT counterions at 100 mM to neutralize charge, for a total of ~16K atoms. HIV peptide simulations were initiated from helical conformations taken from the crystal structure PDB:3V3B. These structures were solvated in a ~ (45 A)3 cubic periodic box with Na+ and CT counterions, for a simulation size of around 9K atoms.

Yet, the application of this approach in the microarray format remains challenging. Fabrication of natural glycoproteins, (Kilcoyne et al., 2012) neoglycopeptides, (Wang et al., 2002) glycodendrimers, (Laigre et al., 2018) DNA-based glycoconjugates, (Hawkes et al., 2019) glycoclusters, (Moni et al., 2009) and glycopolymers (Godula et al., 2009; Zilio et al., 2015) in the microarray format with multivalent presentation require extensive synthetic work prior to the printing onto glass slides. Unfortunately, printing of these compounds on the microarray ties in with solubility and density fluctuations of the material, printing and humidity inconsistencies during coupling, and the microarray surface functionalization (linker) effect, resulting in insufficient coupling and/or poor morphology of the spotted material (Ruprecht et al., 2019; Temme et al., 2019).

Wang, D., Liu, S., Trummer, B. J., Deng, C., and Wang, A. (2002). Carbohydrate Microarrays for the Recognition of Cross-Reactive Molecular Markers of Microbes and Host Cells. Nat. Biotechnol. 20, 275–281. doi:10.1038/nbt0302-275

L58 – Christian Schafmeister, Temple University“Automated Synthesis of Spiroligomers: Programmable, Shape-defined, and Cell-permeable Peptidomimetics”

Happy Holidays from the Wang Lab! We concluded 2022 with some high school outreach -playing with the enzymes & fluorescent probes with students and answering questions related to STEM careers & college life including that. And finally today here is a holiday party!